قائمة الطعام
This work uses upright and inverted cell culture configurations to show that cellular uptake of gold nanoparticles depends on the sedimentation and diffusion velocities of the nanoparticles and is independent of size, shape, density, surface coating and initial concentration. In vitro experiments typically measure the uptake of …
Boulos, S. P. et al. Nanoparticle–protein interactions: a thermodynamic and kinetic study of the adsorption of bovine serum albumin to gold nanoparticle surfaces. Langmuir 29, 14984–14996 (2013).
We study the gravitational sedimentation of citrate- or ascorbate-capped spherical gold nanoparticles (AuNP) by measuring the absorption-vs.-time curve …
Nanoparticles (NPs) suspension is thermodynamically unstable, agglomeration and sedimentation may occur after introducing NPs into a physiological solution, which in turn affects their recognition and uptake by cells. In this work, rod-like gold NPs (AuNRs) with uniform morphology and size were synthesized to study the …
Gold nanoparticles (GNPs) have been synthesized through the citrate reduction method; the citrate/gold(III) ratio was changed from 1:1 up to 10:1 and the size of the resulting nanoparticles was measured by sedimentation field-flow fractionation (SdFFF). Experimental data showed that the GNPs size decreases in the ratio range …
Gold nanoparticles (GNPs) have been synthesized through the citrate reduction method; the citrate/gold(III) ratio was changed from 1:1 up to 10:1 and the size of the resulting nanoparticles was measured by sedimentation field-flow fractionation (SdFFF). Experimental data showed that the GNPs size decreases in the ratio range …
Generally, more nanoparticles are taken up in the upright configuration than in the inverted one, and nanoparticles with faster sedimentation rates showed greater differences in uptake between the two configurations. Our results suggest that sedimentation needs to be considered when performing in vitro studies for large and/or heavy nanoparticles.
The sedimentation cell used has these nanoparticles suspended uniformly to begin with, and because the standard cell for the centrifugation is used, the effects of heating, convection in fluid, etc., are minimized. The physics of centrifugation is modified both at higher concentrations of particles and in highly charged systems.
Sedimentation curves of gold nanoparticles in water were obtained by measuring the optical density of a suspension over time. The results are not subject to sampling errors, and refer to the particles in situ. Curves obtained simultaneously at several wave lengths were analyzed together to derive the size histogram of the sedimenting …
Abstract. Coating the surface of nanoparticles with polyethylene glycol (PEG), or "PEGylation", is a commonly used approach for improving the efficiency of drug and gene delivery to target cells and tissues. Building from the success of PEGylating proteins to improve systemic circulation time and decrease immunogenicity, the impact …
Gold nanoparticles of various shapes, sizes, surface coating, density and initial concentration were examined and those with faster sedimentation rates showed higher cellular uptake in the upright ...
Sedimentation curves of gold nanoparticles in water were obtained by measuring the optical density of a suspension over time. The results are not subject to sampling errors, and refer to the particles in situ. Curves obtained simultaneously at several wave lengths were analyzed together to derive the size histogram of the sedimenting …
Information on shape anisotropy of gold nanoparticles can be deduced from facile VIS-NIR spectroscopy experiments as the spectral position of the longitudinal surface plasmon resonance band is ...
Advances in analytical ultracentrifugation allow for the extraction of the sedimentation (s) and diffusion coefficients (D). ... pentadecanethiol gold nanoparticles (PDT-NPs) synthesized using the ...
The unique properties of gold nanoparticles (AuNPs) make them attractive for use in a number of fields, ranging from cosmetology to medicine. ... 2009), are added to the reaction mixtures to prevent uncontrolled growth and sedimentation of AuNPs. The inclusion of the reducing agents and/or stabilizers not only decreases the purity of the ...
To fill in this research gap, the adsorption of gold nanoparticles on illite was investigated experimentally at a relatively high solid/liquid ratio of 5 g L –1 and the effects of initial pH, ionic strength, citrate concentration, temperature and illite particle size were evaluated. The adsorbed amount of gold nanoparticles (from <5% to ...
Sedimentation and diffusion are important aspects of the behavior of colloidal nanoparticles in solution, and merit attention during the synthesis, characterization, and application of nanoparticles. Here, the sedimentation of nanoparticles is studied quantitatively using digital photography and a simple model …
Here, we use upright and inverted cell culture configurations to show that cellular uptake of gold nanoparticles depends on the sedimentation and diffusion …
Gold nanoparticles (Au NPs) have attracted much attention in recent decades due to their chemical inertness and stability [1], minimum toxicity [2], special and easily tunable optical [3,4] and ...
Sedimentation-diffusion equations were solved for gravitational sedimentation of spherical gold nanoparticles, and there was a good agreement between calculated sedimentation rates and measured ...
Request PDF | Coagulation and sedimentation of gold nanoparticles and illite in model natural waters: Influence of initial particle concentration | Gold nanoparticles (AuNP) possess unique ...
Here, we use upright and inverted cell culture configurations to show that cellular uptake of gold nanoparticles depends on the sedimentation and diffusion velocities of the nanoparticles and is independent of size, shape, density, surface coating and initial concentration of the nanoparticles. Generally, more nanoparticles are taken …
Sedimentation curves for gold nanoparticles in water were obtained by measuring the optical density of a suspension, initially with particle concentration and optical density uniform, over time. As the sedimentation front moved through the optical beam of the instrument, the measured optical density decreased to zero. ...
the sedimentation behaviour of nanoparticle solutions in greater detail. Re-cently, Alexander et al. reported a study of gravitational sedimentation of gold nanoparticles [26]. They focused on measuring the optical density at a pre-de ned height in the solution as a function of time using an UV-visible absorption spectrophotometer.
By culturing cells with gold nanoparticles in both upright and inverted configurations, we demonstrate that in the absence of particle aggregation, the cellular uptake of nanoparticles...
3.2. The role of colloidal stability in nanoparticle sedimentation–diffusion equilibrium. The effect of temperature on the sedimentation–diffusion equilibrium was evaluated by testing 100 nm …
Gold nanoparticles (AuNPs) are one of the most important nanoparticles, and they have been widely used for medical and non-medical applications as ideal …
charged gold nanoparticles in vitro by evaluating their settling dynamics and by developing a ... Gold nanoparticles, sedimentation–diffusion equilibrium, settling dynamics, gravitational
Principle of trapping by a salt gradient in a nanochannel. Our nanofluidic device traps nanoparticles in 16 parallel funnel-shaped nanochannels bridging two microchannels, see Fig. 1a–c ...
We use gold NRs synthesized by a seed-mediated method ( 3) for illustrating the use of centrifugation for shape separation. The size and shape of nanoparticles is gauged by using transmission electron microscopy (TEM) and UV-visible near-infrared (UV-vis-NIR) spectroscopy. In UV-vis spectrum, nanoparticles have size …
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