قائمة الطعام
Product Overview. Documents. The Platinum™ Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR. Designed specifically for endpoint multiplex …
The Platinum™ Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR. Designed specifically for endpoint multiplex PCR, it supports easy multiplexing with minimal optimization. • Multiplexing—Amplify up to 20 amplicons in a single reaction • Flexibility—Amplify products from 50 bp to 2.5 kb
The PlatinumTM Multiplex PCR Master Mix contains all of the components for multiplex end-point PCR (except for primers and templates) in a single tube, including …
Instrument Compatibility TaqMan™ Multiplex Master Mix can be used in fast cycling mode on both standard and fast qPCR instruments, such as: 7500 Real-Time PCR System, 7500 Fast Real-Time PCR System, ViiA™ 7 Real-Time PCR System, and QuantStudio™ 6 Flex, 7 Flex, and 12K Flex Real-Time PCR systems.
Multiplex PCR Master Mix. 2. Mix the Platinum™ Multiplex PCR Master Mix by gently inverting the tube 10 times, then place on ice. IMPORTANT! Avoid bubble formation during mixing. 3. Mix the remaining reagents by inverting each tube a few times, then centrifuge the contents briefly. Place the tubes on ice. Prepare primer mix Platinum ...
The QIAGEN Multiplex PCR Kit is the first kit specifically developed for multiplex PCR and is provided in an easy-to-use master-mix format. QIAGEN Multiplex PCR Master Mix contains preoptimized concentrations of HotStarTaq DNA Polymerase and MgCl 2, plus dNTPs and an innovative PCR buffer specially developed for multiplex PCR.The kit …
A PCR sample should be diluted 2- to 20-fold for optimal separation on E-Gel agarose gels. Reaction components for multiplex PCR Component 20 µL reaction 50 µL reaction Final concentration 5X Platinum II PCR Buffer* 4 µL 10 μL 1X 10 mM dNTP mix 0.4 µL 1 µL 0.2 mM each Forward primers Varies Varies 100–200 nM each
The YFiler Platinum Casework PCR Amplification Kit is a 6-dye multiplex assay that simultaneously amplifies a set of 38 male-specific, Y-chromosome Short Tandem Repeat (YSTR) markers (DYS576, DYS389I, DYS635, DYS389II, DYS627, DYS549, DYS593, DYS645, DYS460, DYS458, DYS19, YGATAH4, DYS448, DYS391, D …
4464269†. Platinum® Multiplex PCR Master Mix, 2, 250 reaction kit. . Store unopened at –15°C to –25°C until the expiration date on the label. After opening, the master mix may be stored at –15°C to –25°C until the expiration date on the label, or at 4°C for up to 30 days. The GC Enhancer must be kept at –15°C to. 25°C.
Platinum 2 ® Multiplex PCR Master Mix 3 Prepare the PCR reaction a. Combine all primers for the multiplex reaction in one tube and adjust the reaction volume to a final concentration of 0.5 µM per primer with 0.1 TE buffer. b. Store aliquots of primer mix at -20 °C for up to a year or 4 °C for up to two months.
Multiplex PCR is a widespread molecular biology technique that enables simultaneous amplification of many targets in a single tube using multiple pairs of primers. Multiplexing using Taq-based DNA polymerases usually requires optimized reaction conditions, so specialized master mixes are often used. This application note describes multiplex PCR ...
The GC Enhancer must be kept at –15°C to –25°C. Platinum® Multiplex PCR Master Mix, 2. . (5 tubes, 1.25 mL each) Store unopened at –15°C to –25°C until the expiration date on the label. After opening, the master mix may be stored at –15°C to –25°C until the expiration date on the label, or at 4°C for up to 30 days.
The YFiler Platinum Casework PCR Amplication Kit is a 6-dye multiplex assay that simultaneously amplies a set of 38 male-specic, Y-chromosome Short Tandem Repeat (YSTR) markers (DYS576,
The Platinum™ Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR. Designed specifically for endpoint multiplex PCR, it supports easy multiplexing …
The Platinum™ Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR. Designed specifically for endpoint multiplex PCR, it supports easy multiplexing …
• 1 x 1.25 mL Platinum Multiplex PCR Master Mix, 2X • 1 x 0.3 mL GC Enhancer Store unopened at –15°C to –25°C. After opening, the master mix may be stored at –15°C to –25°C or at 4°C (for up to 30 days). The GC Enhancer must be kept at –15°C to –25°C. Format: Tube: Polymerase: Platinum Taq DNA Polymerase: Multiplex Capability
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity offers: • High-fidelity end-point detection and analysis of RNA molecules by RT-PCR. • PCR amplification and cDNA synthesis in a single tube. • Detection of a wide range of RNA targets from 300 bp to 10 kb. • Increased specificity with wide cDNA synthesis ...
Platinum 2 ® Multiplex PCR Master Mix 3 Prepare the PCR reaction a. Combine all primers for the multiplex reaction in one tube and adjust the reaction volume to a final …
TaqMan QK Platinum 1-Step Multiplex Master Mix is designed for rapid, multiplex, high-sensitivity real-time quantitative RT-PCR detection with high-quality results for even complex samples. This master mix contains UDG enzyme to help prevent crossover contamination. The enzyme master mix and buffer are provided in concentrated format, …
note describes multiplex PCR using Invitrogen ™ Platinum SuperFi DNA Polymerase. Platinum SuperFi DNA Polymerase is a proofreading enzyme that combines >100x the fidelity of Taq polymerase with trusted Invitrogen ™ Platinum hot-start Table 1. PCR reaction conditions. Component 50 µL reaction Final concentration PCR with master mix
The PlatinumMultiplex PCR Master Mix is designed specifically for endpoint multiplex PCR and supports easy multiplexing with minimal optimization. Multiplexing: Amplify up to 20 amplicons in a single reaction. Flexibility: Amplify products from 50bp to 2.5kb. Ease of Use: Perform multiplex PCR reactions with minimal optimization.
Invitrogen Platinum SuperFi II DNA Polymerase is a hot-start, engineered proofreading DNA polymerase, providing superior fidelity and specificity to your PCR. With >300x Taq fidelity and buffer specially formulated for …
to 15 targets in the same multiplex PCR using Platinum SuperFi DNA Polymerase. With its high robustness and specificity, Platinum SuperFi DNA Polymerase can multiplex over …
Features of Platinum II Hot-Start PCR Master Mix (2X) include: • Innovative buffer —enables universal annealing temperature by isostabilizing primer-template duplex structures. • Engineered Taq DNA polymerase —confers fast cycling and resistance to common inhibitors. • Platinum hot-start technology —enables superior specificity ...
Invitrogen Platinum SuperFi II DNA Polymerase is a hot-start, engineered proofreading DNA polymerase, providing superior fidelity and specificity to your PCR. With >300x Taq fidelity and buffer specially formulated for primer annealing at 60°C, Platinum SuperFi II DNA Polymerase offers efficiency and simplicity in PCR applications requiring highest …
Table 1. PCR reaction conditions. Component 50 µL reaction Final concentration PCR with master mix 2X Platinum SuperFi II PCR Master Mix* 25 µL 1X 10 µM forward primer 1 µL 0.2 µM each 10 µM reverse primer 1 µL 0.2 µM each Template DNA 0.1–250 ng** Varies Water, nuclease-free to 50 µL – PCR with stand-alone enzyme 5X SuperFi II ...
The PlatinumMultiplex PCR Master Mix is designed specifically for endpoint multiplex PCR and supports easy multiplexing with minimal optimization. Multiplexing: Amplify up to 20 …
Platinum ™ SuperFi II PCR Master Mix is a ready-to-use ... Reduce the primer concentration to 0.2 µM final for amplification of >5 kb targets from genomic DNA and for multiplex reactions. Cap each tube, mix, and then briefly centrifuge the contents. 3 Incubate reactions in a thermal cycler (3-step protocol)
Platinum Multiplex PCR Master Mix; No. of amplicons in single reaction : Up to 15-plex: Up to 20-plex: Amplification length: Up to 2.5 kb: Up to 2.5 kb: Hot start for enhanced specificity: Yes: Yes: Fidelity vs. Taq enzyme >300x: 1x: Universal annealing temperature of 60°C: Yes: No: GC-rich amplification: Yes: No: Stand-alone enzyme: Colorless ...
TaqMan QK Platinum Multiple Master Mix is specially designed for fast, multiplex, high-sensitivity real-time PCR detection with high-quality results for even complex samples. This master mix contains UDG enzyme to help prevent crossover contamination, and with high stability and batch-to-batch consistency, it is ideal for biomedical ...
حقوق النشر ©2000- 2024 شركة شنغهاي زينيث
